Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis

Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis

Southern, Edwin M.
Journal of Molecular Biology 98, no. 3 (1975): 503–17
https://doi.org/10.1016/S0022-2836(75)80083-0

“Gels were cast between glass plates (de Wachter & Fiers, 1971). The plates were separated by Perspex side pieces 3 mm thick and along one edge was placed a “comb” of Perspex, which moulded the sample wells in the gel. The Perspex pieces were sealed to the glass plates with silicone grease and the plates clamped together with Bulldog clips. The assembly was stood with the comb along the lower edge. Agarose solution (Sigma electrophoresis grade agarose) was prepared by dissolving the appropriate weight in boiling electrophoresis buffer (E buffer of Loening, 1969). The solution was cooled to 60 to 70°C and poured into the assembly, where it was allowed to set for at least an hour.”

— Edwin M. Southern